SDC3 ELISA Kit

Produktnummer ABIN4884706

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human SDC3. Es wurde in 1 Publikation zitiert.

Kurzübersicht für SDC3 ELISA Kit (ABIN4884706)

Target: SDC3 (Syndecan 3 (SDC3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.061 ng/mL - 15 ng/mL

Applikation: ELISA

Proben: Plasma, Cell Culture Supernatant, Serum

Produktdetails SDC3 ELISA Kit

Untere Nachweisgrenze: 0.061 ng/mL

Verwendungszweck: Human Syndecan-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytische Methode: Quantitative

Spezifität: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS-13, Aggrecan, Angiotensinogen (SERPINA8), B7-H1 (CD274), BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM-1 (CD226), CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine, Galectin-1, GITR Ligand (TNFSF18), Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL-32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Siglec-7 (CD328), Thrombospondin 2, Thrombospondin 5, Tie-1, ULBP-2.

Sensitivität: 60 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2 fold

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Referenzen für SDC3 ELISA Kit (ABIN4884706)

Hudák, Letoha, Letoha: "Peripheral Leukocyte Syndecan-3 Is Elevated in Alzheimer's Disease: Evidence from a Human Study." in: International journal of molecular sciences, Vol. 26, Issue 14, (2025) (PubMed).

Details zu SDC3

Target: SDC3 (Syndecan 3 (SDC3))

Andere Bezeichnung: Syndecan-3

Hintergrund:

Gene Symbols: SDC3, KIAA0468

Protein Name (Synonyms): Syndecan-3 (SYND3)

Gen-ID: 9672

UniProt: O75056

Pathways: Glycosaminoglycan Metabolic Process