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Syntaxin 6 ELISA Kit

STX6 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884683
  • Target Alle Syntaxin 6 (STX6) ELISA Kits anzeigen
    Syntaxin 6 (STX6)
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Syntaxin 6 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle Syntaxin 6 (STX6) ELISA Kits anzeigen
    Syntaxin 6 (STX6)
    Andere Bezeichnung
    Syntaxin 6 (STX6 Produkte)
    Synonyme
    CG7736 ELISA Kit, CT23545 ELISA Kit, Dm Syx6 ELISA Kit, DmSyx6 ELISA Kit, Dmel\\CG7736 ELISA Kit, SYN6 ELISA Kit, SYX6/10 ELISA Kit, Syx ELISA Kit, MGC78971 ELISA Kit, MGC132090 ELISA Kit, STX6 ELISA Kit, fb51b08 ELISA Kit, wu:fb51b08 ELISA Kit, zgc:110658 ELISA Kit, si:dkey-121a11.2 ELISA Kit, LOC734102 ELISA Kit, Syx6 ELISA Kit, Bmsyx6 ELISA Kit, Syntaxin-6 ELISA Kit, 2310039E05Rik ELISA Kit, 2410005I16Rik ELISA Kit, AA437559 ELISA Kit, AI415714 ELISA Kit, AI845861 ELISA Kit, Syntaxin 6 ELISA Kit, syntaxin 6 L homeolog ELISA Kit, syntaxin 6 ELISA Kit, Syx6 ELISA Kit, stx6.L ELISA Kit, STX6 ELISA Kit, stx6 ELISA Kit, Stx6 ELISA Kit
    Gen-ID
    10228
    UniProt
    O43752
    Pathways
    Synaptic Vesicle Exocytosis
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