SIRT5 ELISA Kit

Produktnummer ABIN4884564

Human SIRT5 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human SIRT5.

Kurzübersicht für SIRT5 ELISA Kit (ABIN4884564)

Target: SIRT5 (Sirtuin 5 (SIRT5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.12 ng/mL - 25 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails SIRT5 ELISA Kit

Untere Nachweisgrenze: 0.12 ng/mL

Verwendungszweck: Human Sirtuin?5 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytische Methode: Quantitative

Spezifität: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin RIA, ASAHL, B4GalT1, BAI1, Brorin, C1qTNF4, CA14, CA4, CA6, CA8, Cadherin-6, Caspr2, CD27 Ligand, CD300a, CD300e, CD300f, CD4, CD5, CD69, CK18, CK19, CPB1, CRISP-2, DDR1, FUT8, MIA, NTAL, NTB-A, OMgp, PEAR1, Podoplanin, PTH1R, Reg4, ROR1, Semaphorin 4G, Serpin A5, Serpin B6, Siglec-1, Sirtuin 2.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2 fold

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu SIRT5

Target: SIRT5 (Sirtuin 5 (SIRT5))

Andere Bezeichnung: Sirtuin 5

Hintergrund:

Gene Symbols: SIR2L5, SIRT5

Protein Name (Synonyms): NAD-dependent protein deacylase sirtuin-5, mitochondrial (EC 3.5.1.-) (Regulatory protein SIR2 homolog 5) (SIR2-like protein 5)

Gen-ID: 23408

UniProt: Q9NXA8

Pathways: SARS-CoV-2 Protein Interaktom