CoREST ELISA Kit

Produktnummer ABIN4884324

Human CoREST ELISA Kit, Colorimetric Assay für die Quantifizierung von Human CoREST.

Kurzübersicht für CoREST ELISA Kit (ABIN4884324)

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7 pg/mL - 1500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails CoREST ELISA Kit

Untere Nachweisgrenze: 7 pg/mL

Verwendungszweck: Human RCOR1/CoREST ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytische Methode: Quantitative

Spezifität: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Coagulation Factor X, CHMP2B, Contactin-3, Cortactin, CrkL, Cyr61/CCN1, DAPP1, Dynactin Subunit 1/DCTN1, DFF45/ICAD, DRAK1, GRAP2, GRK5, Hydroxyacid Oxidase-1/HAO-1, LRIG1, MMP-12, NCK1, Nectin-2/CD112, Nesfatin-1/Nucleobindin-2, Neurogranin, Nrf2, NUDT5, PAR1, Pancreatic Polypeptide/PP, Peroxiredoxin 2, PSMA1, PU.1/Spi-1, RalA, Serpin B8, SH2D1A, SHP-1, Siglec-6/CD327, SorCS3, THAP11, ULBP-4/RAET1E, UNC5H3, VAMP-1, VAMP-2, PBEF/Visfatin.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2 fold

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu CoREST

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Andere Bezeichnung: RCOR1

Hintergrund: Gene Symbols: REST corepressor 1 (Protein CoREST)

Gen-ID: 23186

UniProt: Q9UKL0

Pathways: Regulation of Hormone Metabolic Process, Chromatin Binding