Peroxiredoxin 1 ELISA Kit

Produktnummer ABIN4884235

Produktdetails Peroxiredoxin 1 ELISA Kit

Target: Peroxiredoxin 1 (PRDX1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Peroxiredoxin-1/PRDX1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Peroxiredoxin 1

Target: Peroxiredoxin 1 (PRDX1)

Andere Bezeichnung: Peroxiredoxin-1 (PRDX1 Produkte)

Synonyme: MSP23 ELISA Kit, NKEF-A ELISA Kit, NKEFA ELISA Kit, PAG ELISA Kit, PAGA ELISA Kit, PAGB ELISA Kit, PRX1 ELISA Kit, PRXI ELISA Kit, TDPX2 ELISA Kit, NkefA ELISA Kit, OSF-3 ELISA Kit, OSF3 ELISA Kit, Paga ELISA Kit, PrdxI ELISA Kit, PrxI ELISA Kit, TDX2 ELISA Kit, TPxA ELISA Kit, Tdpx2 ELISA Kit, prx1 ELISA Kit, Hbp23 ELISA Kit, MGC80194 ELISA Kit, MGC84820 ELISA Kit, PRDX1 ELISA Kit, msp23 ELISA Kit, nkefa ELISA Kit, pag ELISA Kit, paga ELISA Kit, pagb ELISA Kit, prx-1 ELISA Kit, prxi ELISA Kit, tdpx2 ELISA Kit, hm:zehl0637 ELISA Kit, zgc:110343 ELISA Kit, TPX-2 ELISA Kit, peroxiredoxin 1 ELISA Kit, peroxiredoxin 1 S homeolog ELISA Kit, peroxiredoxin-1 pseudogene ELISA Kit, PRDX1 ELISA Kit, Prdx1 ELISA Kit, prdx1.S ELISA Kit, prdx1 ELISA Kit, LOC100350232 ELISA Kit

Gen-ID: 5052

UniProt: Q06830

Pathways: p53 Signalweg, EGFR Signaling Pathway, CXCR4-mediated Signaling Events