PRCP ELISA Kit

Produktnummer ABIN4884229

Produktdetails PRCP ELISA Kit

Target: PRCP (Prolylcarboxypeptidase (PRCP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human PRCP ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu PRCP

Target: PRCP (Prolylcarboxypeptidase (PRCP))

Andere Bezeichnung: PRCP (PRCP Produkte)

Synonyme: OI13 ELISA Kit, PCOLC ELISA Kit, PCP ELISA Kit, PCP2 ELISA Kit, TLD ELISA Kit, humpcp ELISA Kit, MGC145178 ELISA Kit, 2510048K03Rik ELISA Kit, 2610104A14Rik ELISA Kit, AI451719 ELISA Kit, HUMPCP ELISA Kit, zgc:91816 ELISA Kit, bone morphogenetic protein 1 ELISA Kit, prolylcarboxypeptidase ELISA Kit, lysosomal Pro-X carboxypeptidase ELISA Kit, prolylcarboxypeptidase (angiotensinase C) ELISA Kit, BMP1 ELISA Kit, prcp ELISA Kit, LOC5579822 ELISA Kit, CpipJ_CPIJ006123 ELISA Kit, CpipJ_CPIJ006124 ELISA Kit, Prcp ELISA Kit, PRCP ELISA Kit

Gen-ID: 5547

UniProt: P42785