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Plexin A4 ELISA Kit

PLXNA4 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884197
  • Target Alle Plexin A4 (PLXNA4) ELISA Kits anzeigen
    Plexin A4 (PLXNA4)
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Plexin A4 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle Plexin A4 (PLXNA4) ELISA Kits anzeigen
    Plexin A4 (PLXNA4)
    Andere Bezeichnung
    Plexin A4 (PLXNA4 Produkte)
    Synonyme
    D204 ELISA Kit, wu:fd49b01 ELISA Kit, wu:fe15f03 ELISA Kit, PLXNA4A ELISA Kit, FAYV2820 ELISA Kit, PLEXA4 ELISA Kit, PLXNA4B ELISA Kit, PRO34003 ELISA Kit, 9330117B14 ELISA Kit, Plxa4 ELISA Kit, mKIAA1550 ELISA Kit, PLEX2 ELISA Kit, Plxna4 ELISA Kit, PLXNA4 ELISA Kit, plexin A4 ELISA Kit, plexin-A4 ELISA Kit, plxna4 ELISA Kit, PLXNA4 ELISA Kit, LOC100467706 ELISA Kit, Plxna4 ELISA Kit
    Gen-ID
    91584
    UniProt
    Q9HCM2
    Pathways
    Regulation of Cell Size
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