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NRF2 ELISA Kit
NFE2L2
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4883982
Produktdetails NRF2 ELISA Kit
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Target
Alle NRF2 (NFE2L2) ELISA Kits anzeigen
NRF2 (NFE2L2)
(Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
Reaktivität
Alle Reaktivitäten für NRF2 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Human NRF2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Human NRF2.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu NRF2
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Target
Alle NRF2 (NFE2L2) ELISA Kits anzeigen
NRF2 (NFE2L2)
(Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
Andere Bezeichnung
Nrf2 (NFE2L2 Produkte )
Synonyme
Nrf2 ELISA Kit, nfe2l2 ELISA Kit, wu:fc15g09 ELISA Kit, wu:fj67e03 ELISA Kit, NF-E2R2 ELISA Kit, NRF2 ELISA Kit, AI194320 ELISA Kit, nuclear factor, erythroid 2-like 2a ELISA Kit, nuclear factor, erythroid 2 like 2 S homeolog ELISA Kit, nuclear factor, erythroid 2 like 2 ELISA Kit, nuclear factor, erythroid derived 2, like 2 ELISA Kit, nuclear factor, erythroid 2-like 2 ELISA Kit, nfe2l2a ELISA Kit, nfe2l2.S ELISA Kit, NFE2L2 ELISA Kit, Nfe2l2 ELISA Kit
Gen-ID
4780
UniProt
Q16236
Pathways
ER-Nucleus Signaling , Negative Regulation of intrinsic apoptotic Signaling
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