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NPTXR ELISA Kit

NPTXR Reaktivität: Human Colorimetric Sandwich ELISA 0.410-100 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4883974
  • Target Alle NPTXR ELISA Kits anzeigen
    NPTXR (Neuronal Pentraxin Receptor (NPTXR))
    Reaktivität
    • 3
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.410-100 ng/mL
    Untere Nachweisgrenze
    0.410 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human NPTXR ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human NPTXR.
    Sensitivität
    0.4 ng/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product NPTXR ELISA Kit
  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle NPTXR ELISA Kits anzeigen
    NPTXR (Neuronal Pentraxin Receptor (NPTXR))
    Andere Bezeichnung
    NPTXR (NPTXR Produkte)
    Synonyme
    NPR ELISA Kit, 1200009K17Rik ELISA Kit, 1700036C17Rik ELISA Kit, 5730406O18Rik ELISA Kit, AI452369 ELISA Kit, AI785356 ELISA Kit, D15Bwg0580e ELISA Kit, Npcd ELISA Kit, Npr ELISA Kit, npr ELISA Kit, nptxr ELISA Kit, si:dkeyp-20e4.1 ELISA Kit, zgc:158178 ELISA Kit, neuronal pentraxin receptor ELISA Kit, neuronal pentraxin receptor L homeolog ELISA Kit, neuronal pentraxin receptor b ELISA Kit, NPTXR ELISA Kit, Nptxr ELISA Kit, nptxr.L ELISA Kit, nptxrb ELISA Kit
    Gen-ID
    23467
    UniProt
    O95502
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