NKp44/NCR2 ELISA Kit

Produktnummer ABIN4883931

Human NKp44/NCR2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human NKp44/NCR2.

Kurzübersicht für NKp44/NCR2 ELISA Kit (ABIN4883931)

Target: NKp44/NCR2 (NCR2) (Natural Cytotoxicity Triggering Receptor 2 (NCR2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 13 pg/mL - 3000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails NKp44/NCR2 ELISA Kit

Untere Nachweisgrenze: 13 pg/mL

Verwendungszweck: Human NKp44 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytische Methode: Quantitative

Spezifität: This ELISA kit shows no cross-reactivity with the following cytokines tested: human B7-2 (CD86), BAFF R (TNFRSF13C), Calcitonin, Calsyntenin-1, Cathepsin E, cIAP-2 (HIAP-1), Coagulation Factor VII, Complement MASP3, Endocan, EphA2, EphB4, Ephrin-A4, FGF-23, FGF-5, Flt-3 (Flk-2), GLP-1 (7-37 & 7-36- NH2), Glypican 2, GM-CSF R alpha, GP73 (GOLM1), HTRA2 (Omi), IL-20 R alpha, IL-4 R alpha, JAM-C, Luteinizing Hormone (LH), Matrilin-3, Meprin alpha (MEP1A), MSP R (Ron), N-Cadherin, Neprilysin-2 (MMEL1), Pappalysin-1 (PAPP-A), Parathyroid hormone (PTH), Pepsinogen II (PGC), Peptide YY (PYY), Presenilin 1 (PSEN1), SOX2, TFPI-2, TRACP (ACP5), Trefoil factor 3 (TFF3), Ubiquitin+1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2 fold

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu NKp44/NCR2

Target: NKp44/NCR2 (NCR2) (Natural Cytotoxicity Triggering Receptor 2 (NCR2))

Andere Bezeichnung: NKp44

Hintergrund: Gene Symbols: Natural cytotoxicity triggering receptor 2 (Lymphocyte antigen 95 homolog) (NK cell-activating receptor) (Natural killer cell p44-related protein) (NK-p44) (NKp44) (CD antigen CD336)

Gen-ID: 9436

UniProt: O95944