METAP2 ELISA Kit

Produktnummer ABIN4883772

Produktdetails METAP2 ELISA Kit

Target: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human METAP2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu METAP2

Target: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Andere Bezeichnung: METAP2 (METAP2 Produkte)

Synonyme: metap2 ELISA Kit, MGC53792 ELISA Kit, METAP2 ELISA Kit, GB13458 ELISA Kit, DDBDRAFT_0190923 ELISA Kit, DDBDRAFT_0304991 ELISA Kit, DDB_0190923 ELISA Kit, DDB_0304991 ELISA Kit, 4930584B20Rik ELISA Kit, A930035J23Rik ELISA Kit, AI047573 ELISA Kit, AL024412 ELISA Kit, AU014659 ELISA Kit, Amp2 ELISA Kit, Mnpep ELISA Kit, p67 ELISA Kit, p67eIF2 ELISA Kit, MAP2 ELISA Kit, MNPEP ELISA Kit, wu:fb98h06 ELISA Kit, zgc:66250 ELISA Kit, methionyl aminopeptidase 2 L homeolog ELISA Kit, methionyl aminopeptidase 2 ELISA Kit, methionine aminopeptidase 2 ELISA Kit, methionine aminopeptidase ELISA Kit, methionyl aminopeptidase 2 S homeolog ELISA Kit, methionyl aminopeptidase 2b ELISA Kit, metap2.L ELISA Kit, METAP2 ELISA Kit, metap2 ELISA Kit, LOC551771 ELISA Kit, CAALFM_C604080WA ELISA Kit, Metap2 ELISA Kit, metap2.S ELISA Kit, metap2b ELISA Kit

Gen-ID: 10988

UniProt: P50579

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling