Lactoferrin ELISA Kit

Produktnummer ABIN4883682

Produktdetails Lactoferrin ELISA Kit

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.96-480 ng/mL

Untere Nachweisgrenze: 1.96 ng/mL

Applikation: ELISA

Verwendungszweck: Human Lactoferrin ELISA Kit for serum, plasma, and cell culture supernatant samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adipsin, Angiogenin, B2M, bIG-H3, CD14, CRP, DPPIV, Endostatin, E-Selectin, FAP, Ferritin, Fetuin A, gp130, HGFR, ICAM-2, IGFBP-2, IGFBP-3, IGFBP-6, Lipocalin-2, L-Selectin, LYVE-1, MCSF R, MMP-9, NCAM-1, Nidogen-1, PAI-1, PF4, RBP4, SAA, TSP-1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Andere Bezeichnung: Lactoferrin (LTF Produkte)

Synonyme: LTF ELISA Kit, GIG12 ELISA Kit, HLF2 ELISA Kit, LF ELISA Kit, Lf ELISA Kit, Csp82 ELISA Kit, MMS10R ELISA Kit, Ms10r ELISA Kit, PLF ELISA Kit, TFEW ELISA Kit, conalbumin ELISA Kit, lactotransferrin ELISA Kit, transferrin ELISA Kit, transferrin (ovotransferrin) ELISA Kit, LTF ELISA Kit, CpipJ_CPIJ000114 ELISA Kit, TF ELISA Kit, Ltf ELISA Kit

Gen-ID: 4057

UniProt: P02788

Pathways: Transition Metal Ion Homeostasis