PSIP1 ELISA Kit

Produktnummer ABIN4883589

Produktdetails PSIP1 ELISA Kit

Target: PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human LEDGF ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu PSIP1

Target: PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))

Andere Bezeichnung: LEDGF (PSIP1 Produkte)

Synonyme: PSIP1 ELISA Kit, im:7149861 ELISA Kit, zgc:109898 ELISA Kit, LEDGF ELISA Kit, p75 ELISA Kit, PSIP2 ELISA Kit, DFS70 ELISA Kit, PAIP ELISA Kit, p52 ELISA Kit, AA408851 ELISA Kit, AU015605 ELISA Kit, Dfs70 ELISA Kit, Ledgfa ELISA Kit, Ledgfb ELISA Kit, Psip2 ELISA Kit, Ledgf ELISA Kit, SBP75 ELISA Kit, LEDGF/p75 ELISA Kit, PC4 and SFRS1 interacting protein 1 S homeolog ELISA Kit, PC4 and SFRS1 interacting protein 1 ELISA Kit, PC4 and SFRS1 interacting protein 1a ELISA Kit, LEDGF/p75 transcription factor-like ELISA Kit, psip1.S ELISA Kit, PSIP1 ELISA Kit, psip1a ELISA Kit, psip1 ELISA Kit, Psip1 ELISA Kit

Gen-ID: 11168

UniProt: O75475

Pathways: Chromatin Binding, Ribonucleoprotein Complex Subunit Organization