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KIRREL3 ELISA Kit

KIRREL3 Reaktivität: Human Colorimetric Sandwich ELISA 0.21-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4883512
  • Target Alle KIRREL3 ELISA Kits anzeigen
    KIRREL3 (Kin of IRRE Like 3 (KIRREL3))
    Reaktivität
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.21-50 ng/mL
    Untere Nachweisgrenze
    0.21 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human KIRREL3 (NEPH2) ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand, Activin RIIB, Aminopeptidase P2, BAMBI, BOC, Brevican, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320 (TCblR, 8D6A), CDNF, CDO, CHST1, CHST4, CILP-1, CNTF R alpha, CRIM1, CRTAC1, CXADR, Dopa Decarboxylase, DPPII, DSPG3, EMR2, FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Gas6, GPR56, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, KLF4, LAIR1, LAMP, LAMP1 (CD107a),MDGA1, MIS RII, Neurexin
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle KIRREL3 ELISA Kits anzeigen
    KIRREL3 (Kin of IRRE Like 3 (KIRREL3))
    Andere Bezeichnung
    KIRREL3///NEPH2 (KIRREL3 Produkte)
    Synonyme
    KIRRE ELISA Kit, MRD4 ELISA Kit, NEPH2 ELISA Kit, PRO4502 ELISA Kit, 1500010O20Rik ELISA Kit, 2900036G11Rik ELISA Kit, SST4 ELISA Kit, mKIAA1867 ELISA Kit, kirre like nephrin family adhesion molecule 3 ELISA Kit, kin of IRRE like 3 (Drosophila) ELISA Kit, KIRREL3 ELISA Kit, Kirrel3 ELISA Kit
    Gen-ID
    84623
    UniProt
    Q8IZU9
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