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IL27 Receptor alpha ELISA Kit

IL27RA Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883399
  • Target Alle IL27 Receptor alpha (IL27RA) ELISA Kits anzeigen
    IL27 Receptor alpha (IL27RA) (Interleukin 27 Receptor, alpha (IL27RA))
    Reaktivität
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human IL-27 Receptor alpha (IL27RA) ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Human IL-27 Receptor alpha (IL27RA)
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle IL27 Receptor alpha (IL27RA) ELISA Kits anzeigen
    IL27 Receptor alpha (IL27RA) (Interleukin 27 Receptor, alpha (IL27RA))
    Andere Bezeichnung
    IL-27 R alpha (IL27RA Produkte)
    Synonyme
    IL27RA ELISA Kit, CRL1 ELISA Kit, IL-27RA ELISA Kit, IL27R ELISA Kit, TCCR ELISA Kit, WSX1 ELISA Kit, zcytor1 ELISA Kit, IL-27R ELISA Kit, Tccr ELISA Kit, WSX-1 ELISA Kit, Wsx1 ELISA Kit, interleukin 27 receptor subunit alpha ELISA Kit, interleukin-27 receptor subunit alpha ELISA Kit, interleukin 27 receptor, alpha ELISA Kit, IL27RA ELISA Kit, LOC484907 ELISA Kit, Il27ra ELISA Kit
    Gen-ID
    9466
    UniProt
    Q6UWB1
    Pathways
    Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response
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