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IL17RD ELISA Kit

IL17RD Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883328
  • Target Alle IL17RD ELISA Kits anzeigen
    IL17RD (Interleukin 17 Receptor D (IL17RD))
    Reaktivität
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human IL-17 RD ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes human IL-17 RD. Other species not yet determined.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product IL17RD ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle IL17RD ELISA Kits anzeigen
    IL17RD (Interleukin 17 Receptor D (IL17RD))
    Andere Bezeichnung
    IL-17 RD (IL17RD Produkte)
    Synonyme
    IL17RD ELISA Kit, sef ELISA Kit, il-17rd ELISA Kit, il17rlm ELISA Kit, HH18 ELISA Kit, IL-17RD ELISA Kit, IL17RLM ELISA Kit, SEF ELISA Kit, 2810004A10Rik ELISA Kit, AI428510 ELISA Kit, Sef ELISA Kit, Sef-S ELISA Kit, RGD1565237 ELISA Kit, cb208 ELISA Kit, id:ibd2875 ELISA Kit, id:ibd5059 ELISA Kit, zgc:136328 ELISA Kit, interleukin 17 receptor D ELISA Kit, IL17RD ELISA Kit, il17rd ELISA Kit, Il17rd ELISA Kit
    Gen-ID
    54756
    UniProt
    Q8NFM7
    Pathways
    Toll-Like Receptors Cascades
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