HRG ELISA Kit

Produktnummer ABIN4883212

Produktdetails HRG ELISA Kit

Target: HRG (Histidine-Rich Glycoprotein (HRG))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.23-300 ng/mL

Untere Nachweisgrenze: 1.23 ng/mL

Applikation: ELISA

Verwendungszweck: Human HPRG ELISA Kit for serum, plasma, and cell culture supernatant samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human HPRG. Other species not determined.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples1,000 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu HRG

Target: HRG (Histidine-Rich Glycoprotein (HRG))

Andere Bezeichnung: HPRG (HRG Produkte)

Synonyme: AI265597 ELISA Kit, AW413091 ELISA Kit, D16jh2 ELISA Kit, D18020 ELISA Kit, Hprg ELISA Kit, Hrgp ELISA Kit, HPRG ELISA Kit, HRGP ELISA Kit, THPH11 ELISA Kit, HRG1 ELISA Kit, histidine rich glycoprotein ELISA Kit, histidine-rich glycoprotein ELISA Kit, HRG ELISA Kit, LOAG_12427 ELISA Kit, LOC100597044 ELISA Kit, Hrg ELISA Kit

Gen-ID: 3273

UniProt: P04196