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HNF4A ELISA Kit
HNF4A
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883203
Produktdetails HNF4A ELISA Kit
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Target
Alle HNF4A ELISA Kits anzeigen
HNF4A
(Hepatocyte Nuclear Factor 4, alpha (HNF4A))
Reaktivität
Alle Reaktivitäten für HNF4A ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human HNF-4 alpha/NR2A1 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes .
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu HNF4A
(ausblenden)
Target
Alle HNF4A ELISA Kits anzeigen
HNF4A
(Hepatocyte Nuclear Factor 4, alpha (HNF4A))
Andere Bezeichnung
HNF-4 alpha (HNF4A Produkte )
Synonyme
HNF4A ELISA Kit, HNF4alpha ELISA Kit, CG9310 ELISA Kit, DmHNF4 ELISA Kit, Dmel\\CG9310 ELISA Kit, HNF-4 ELISA Kit, HNF-4(D) ELISA Kit, HNF4 ELISA Kit, Hnf-4 ELISA Kit, Hnf-4h ELISA Kit, NR2A4 ELISA Kit, dHNF-4 ELISA Kit, dHNF4 ELISA Kit, Hnf4 ELISA Kit, Hnf4alpha ELISA Kit, MODY1 ELISA Kit, Nr2a1 ELISA Kit, Tcf14 ELISA Kit, HNF4a7 ELISA Kit, HNF4a8 ELISA Kit, HNF4a9 ELISA Kit, MODY ELISA Kit, NR2A1 ELISA Kit, NR2A21 ELISA Kit, TCF ELISA Kit, TCF14 ELISA Kit, HNF-4alpha ELISA Kit, fb58h09 ELISA Kit, id:ibd1279 ELISA Kit, wu:fb58h09 ELISA Kit, hnf4 ELISA Kit, nr2a1 ELISA Kit, hepatocyte nuclear factor 4 alpha ELISA Kit, Hepatocyte nuclear factor 4 ELISA Kit, hepatic nuclear factor 4, alpha ELISA Kit, hepatocyte nuclear factor 4, alpha ELISA Kit, hepatocyte nuclear factor 4 alpha S homeolog ELISA Kit, HNF4A ELISA Kit, hnf4a ELISA Kit, Hnf4 ELISA Kit, Hnf4a ELISA Kit, hnf4a.S ELISA Kit
Gen-ID
3172
UniProt
P41235
Pathways
AMPK Signaling , Nuclear Receptor Transcription Pathway , Steroid Hormone Mediated Signaling Pathway , Carbohydrate Homeostasis , Cell-Cell Junction Organization , Regulation of Carbohydrate Metabolic Process
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