HMGB3 ELISA Kit

Produktnummer ABIN4883200

Produktdetails HMGB3 ELISA Kit

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human HMGB3 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu HMGB3

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Andere Bezeichnung: HMGB3 (HMGB3 Produkte)

Synonyme: HMG-2a ELISA Kit, HMG-4 ELISA Kit, HMG2A ELISA Kit, HMG4 ELISA Kit, Hmg2a ELISA Kit, Hmg4 ELISA Kit, RGD1564407 ELISA Kit, hmgb3 ELISA Kit, MGC54022 ELISA Kit, Xhmgb3 ELISA Kit, MGC88931 ELISA Kit, fa19b06 ELISA Kit, fj43d02 ELISA Kit, wu:fa19b06 ELISA Kit, wu:fj43d02 ELISA Kit, zgc:112073 ELISA Kit, HMGB3 ELISA Kit, NFD03 ELISA Kit, NFD3 ELISA Kit, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR ELISA Kit, high mobility group B3 ELISA Kit, HMG-1 ELISA Kit, HMG2a ELISA Kit, HMGB1 ELISA Kit, high mobility group box 3 ELISA Kit, high mobility group box 3b ELISA Kit, high mobility group box 3 S homeolog ELISA Kit, high mobility group protein ELISA Kit, high mobility group B3 ELISA Kit, High mobility group protein B3 ELISA Kit, HMGB3 ELISA Kit, Hmgb3 ELISA Kit, hmgb3 ELISA Kit, hmgb3b ELISA Kit, hmgb3.S ELISA Kit

Gen-ID: 3149

UniProt: O15347