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GLO1 ELISA Kit
GLO1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883094
Produktdetails GLO1 ELISA Kit
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Target
Alle GLO1 ELISA Kits anzeigen
GLO1
(Glyoxalase I (GLO1))
Reaktivität
Alle Reaktivitäten für GLO1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human Glyoxalase I ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes .
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu GLO1
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Target
Alle GLO1 ELISA Kits anzeigen
GLO1
(Glyoxalase I (GLO1))
Andere Bezeichnung
Glyoxalase I (GLO1 Produkte )
Synonyme
GLOD1 ELISA Kit, GLYI ELISA Kit, 0610009E22Rik ELISA Kit, 1110008E19Rik ELISA Kit, 2510049H23Rik ELISA Kit, AW550643 ELISA Kit, GLY1 ELISA Kit, Glo-1 ELISA Kit, Glo-1r ELISA Kit, Glo-1s ELISA Kit, Glo1-r ELISA Kit, Glo1-s ELISA Kit, Qglo ELISA Kit, cb554 ELISA Kit, zgc:66035 ELISA Kit, wu:fb82g09 ELISA Kit, glod1 ELISA Kit, glyi ELISA Kit, glo1 ELISA Kit, GLO1 ELISA Kit, ATGLX1 ELISA Kit, F12F1.32 ELISA Kit, F12F1_32 ELISA Kit, GLYOXALASE I ELISA Kit, glyoxalase I homolog ELISA Kit, trypanothione-dependent glyoxalase I ELISA Kit, glyoxalase I ELISA Kit, glyoxalase 1 ELISA Kit, glyoxalase 1 L homeolog ELISA Kit, glyoxalase 1 S homeolog ELISA Kit, lactoylglutathione lyase ELISA Kit, glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein ELISA Kit, GLO1 ELISA Kit, Glo1 ELISA Kit, glo1 ELISA Kit, glo1.L ELISA Kit, glo1.S ELISA Kit, GST3 ELISA Kit, GLX1 ELISA Kit, STY1687 ELISA Kit, Bcen_2094 ELISA Kit, gloA ELISA Kit
Gen-ID
2739
UniProt
Q04760
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