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FCRL5 ELISA Kit

FCRL5 Reaktivität: Human Colorimetric Sandwich ELISA 1.64-400 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4882841
  • Target Alle FCRL5 ELISA Kits anzeigen
    FCRL5 (Fc Receptor-Like 5 (FCRL5))
    Reaktivität
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.64-400 ng/mL
    Untere Nachweisgrenze
    1.64 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human FCRL5/FcRH5 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human FCRL5.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product FCRL5 ELISA Kit
  • Applikationshinweise
    Recommended Dilution for serum and plasma samples3 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle FCRL5 ELISA Kits anzeigen
    FCRL5 (Fc Receptor-Like 5 (FCRL5))
    Andere Bezeichnung
    FCRL5 (FCRL5 Produkte)
    Synonyme
    FCRL5 ELISA Kit, BXMAS1 ELISA Kit, CD307 ELISA Kit, CD307e ELISA Kit, FCRH5 ELISA Kit, IRTA2 ELISA Kit, PRO820 ELISA Kit, Fcrh3 ELISA Kit, mBXMH2 ELISA Kit, Fc receptor like 5 ELISA Kit, Fc receptor-like 5 ELISA Kit, FCRL5 ELISA Kit, Fcrl5 ELISA Kit
    Gen-ID
    83416
    UniProt
    Q96RD9
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