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Desmin ELISA Kit

DES Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4882602
  • Target Alle Desmin (DES) ELISA Kits anzeigen
    Desmin (DES)
    Reaktivität
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Desmin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human Desmin.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle Desmin (DES) ELISA Kits anzeigen
    Desmin (DES)
    Andere Bezeichnung
    Desmin (DES Produkte)
    Synonyme
    DES ELISA Kit, des-b ELISA Kit, MGC80853 ELISA Kit, des ELISA Kit, csm1 ELISA Kit, csm2 ELISA Kit, desm ELISA Kit, cmd1i ELISA Kit, MGC75911 ELISA Kit, LOC100220724 ELISA Kit, desmin ELISA Kit, cb290 ELISA Kit, fb59a12 ELISA Kit, wu:fb59a12 ELISA Kit, zgc:109859 ELISA Kit, CSM1 ELISA Kit, CSM2 ELISA Kit, LGMD2R ELISA Kit, wu:fc11d08 ELISA Kit, zgc:154009 ELISA Kit, des-a ELISA Kit, MGC52614 ELISA Kit, desmin ELISA Kit, desmin, gene 1 L homeolog ELISA Kit, desmin, gene 1 ELISA Kit, desmin a ELISA Kit, desmin b ELISA Kit, desmin, gene 1 S homeolog ELISA Kit, desmin, gene 2 S homeolog ELISA Kit, DES ELISA Kit, des.1.L ELISA Kit, des.1 ELISA Kit, des ELISA Kit, desma ELISA Kit, Des ELISA Kit, desmb ELISA Kit, des.1.S ELISA Kit, des.2.S ELISA Kit
    Gen-ID
    1674
    UniProt
    P17661
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