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CRTAC1 ELISA Kit
CRTAC1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4882490
Produktdetails CRTAC1 ELISA Kit
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Target
Alle CRTAC1 ELISA Kits anzeigen
CRTAC1
(Cartilage Acidic Protein 1 (CRTAC1))
Reaktivität
Alle Reaktivitäten für CRTAC1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human CRTAC1 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Human CRTAC1. Other species not yet determined.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu CRTAC1
(ausblenden)
Target
Alle CRTAC1 ELISA Kits anzeigen
CRTAC1
(Cartilage Acidic Protein 1 (CRTAC1))
Andere Bezeichnung
CRTAC1 (CRTAC1 Produkte )
Synonyme
ASPIP ELISA Kit, cb184 ELISA Kit, crtac1 ELISA Kit, sb:cb184 ELISA Kit, zgc:165343 ELISA Kit, aspic1 ELISA Kit, cep-68 ELISA Kit, MGC146658 ELISA Kit, ASPIC ELISA Kit, ASPIC1 ELISA Kit, CEP-68 ELISA Kit, 2810454P21Rik ELISA Kit, AW047536 ELISA Kit, Crtac1B ELISA Kit, Lotus ELISA Kit, W307 ELISA Kit, cartilage acidic protein 1 ELISA Kit, cartilage acidic protein 1a ELISA Kit, CRTAC1 ELISA Kit, crtac1a ELISA Kit, crtac1 ELISA Kit, aspip ELISA Kit, Crtac1 ELISA Kit
Gen-ID
55118
UniProt
Q9NQ79
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