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CRHBP ELISA Kit
CRHBP
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4882475
Produktdetails CRHBP ELISA Kit
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Target
Alle CRHBP ELISA Kits anzeigen
CRHBP
(Corticotropin Releasing Hormone Binding Protein (CRHBP))
Reaktivität
Alle Reaktivitäten für CRHBP ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human CRHBP ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu CRHBP
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Target
Alle CRHBP ELISA Kits anzeigen
CRHBP
(Corticotropin Releasing Hormone Binding Protein (CRHBP))
Andere Bezeichnung
CRHBP (CRHBP Produkte )
Synonyme
crh-BP ELISA Kit, GB14094 ELISA Kit, zgc:91905 ELISA Kit, CRHBP ELISA Kit, CRH-BP ELISA Kit, CRF-BP ELISA Kit, CRFBP ELISA Kit, Crfbp ELISA Kit, crhbp-A ELISA Kit, gene E ELISA Kit, corticotropin releasing hormone binding protein ELISA Kit, corticotropin releasing hormone binding protein S homeolog ELISA Kit, CRHBP ELISA Kit, crhbp ELISA Kit, crh-BP ELISA Kit, Crhbp ELISA Kit, crhbp.S ELISA Kit
Gen-ID
1393
UniProt
P24387
Pathways
Negative Regulation of Hormone Secretion
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