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Calsyntenin 1 ELISA Kit

CLSTN1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4882387
  • Target Alle Calsyntenin 1 (CLSTN1) ELISA Kits anzeigen
    Calsyntenin 1 (CLSTN1)
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Calsyntenin-1 (Alcadein-alpha) ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes human Calsyntenin-1. Other species not yet determined.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle Calsyntenin 1 (CLSTN1) ELISA Kits anzeigen
    Calsyntenin 1 (CLSTN1)
    Andere Bezeichnung
    Calsyntenin-1 (CLSTN1 Produkte)
    Synonyme
    CALSYNTENIN-1 ELISA Kit, fm67g03 ELISA Kit, wu:fk52g02 ELISA Kit, wu:fm67g03 ELISA Kit, zgc:153744 ELISA Kit, zgc:56528 ELISA Kit, clstn1 ELISA Kit, 1810034E21Rik ELISA Kit, Cst-1 ELISA Kit, Cstn1 ELISA Kit, ALC-ALPHA ELISA Kit, CDHR12 ELISA Kit, CSTN1 ELISA Kit, PIK3CD ELISA Kit, XB31alpha ELISA Kit, alcalpha1 ELISA Kit, alcalpha2 ELISA Kit, calsyntenin 1 ELISA Kit, calsyntenin 3 ELISA Kit, beta-catenin-interacting protein 1 ELISA Kit, calsyntenin-1 ELISA Kit, CLSTN1 ELISA Kit, clstn1 ELISA Kit, clstn3 ELISA Kit, LOC100356725 ELISA Kit, LOC100435584 ELISA Kit, Clstn1 ELISA Kit, LOC479600 ELISA Kit
    Gen-ID
    22883
    UniProt
    O94985
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