CILP ELISA Kit

Produktnummer ABIN4882357

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human CILP. Es wurde in 1 Publikation zitiert.

Kurzübersicht für CILP ELISA Kit (ABIN4882357)

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.053 ng/mL - 13 ng/mL

Applikation: ELISA

Proben: Plasma, Cell Culture Supernatant, Serum

Produktdetails CILP ELISA Kit

Untere Nachweisgrenze: 0.053 ng/mL

Verwendungszweck: Human CILP-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytische Methode: Quantitative

Spezifität: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand, Activin RIIB, Aminopeptidase P2, BAMBI, BOC, Brevican, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320, CDNF, CDO, CHST1, CHST4, CNTF R alpha, CRIM1, CRTAC1, CXADR, Dopa Decarboxylase (DDC), DPPII (QPP, DPP7), DSPG3, EMR2, FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Gas6, GPR56, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, Kirrel3 (NEPH2), KLF4, LAIR1, LAMP, LAMP1 (CD107a), MDGA1, MIS RII, Neurexin 3.

Sensitivität: 0.05 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2 fold

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Referenzen für CILP ELISA Kit (ABIN4882357)

Weidenhammer, Prausmüller, Partsch, Spinka, Luckerbauer, Larch, Arfsten, Abdel Mawgoud, Bartko, Goliasch, Kastl, Hengstenberg, Hülsmann, Pavo: "CILP-1 Is a Biomarker for Backward Failure and Right Ventricular Dysfunction in HFrEF." in: Cells, Vol. 12, Issue 24, (2024) (PubMed).

Details zu CILP

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Andere Bezeichnung: CILP-1

Hintergrund:

Gene Symbols: CILP, UNQ602, PRO1188

Protein Name (Synonyms): Cartilage intermediate layer protein 1 (CILP-1) (Cartilage intermediate-layer protein) [Cleaved into: Cartilage intermediate layer protein 1 C1 Cartilage intermediate layer protein 1 C2

Gen-ID: 8483

UniProt: O75339