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ENTPD3 ELISA Kit

Human ENTPD3 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human ENTPD3.
Produktnummer ABIN4882200

Kurzübersicht für ENTPD3 ELISA Kit (ABIN4882200)

Target

Alle ENTPD3 ELISA Kits anzeigen
ENTPD3 (Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3))

Reaktivität

  • 2
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Applikation

ELISA

Proben

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
  • Verwendungszweck

    Custom Human CD39L3 ELISA Kit.

    Analytische Methode

    Quantitative

    Spezifität

    This ELISA antibody pair recognizes Human Gastrokine-1.

    Produktmerkmale

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Benötigtes Material

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    -20 °C

    Informationen zur Lagerung

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Haltbarkeit

    6 months
  • Target Alle ENTPD3 ELISA Kits anzeigen

    ENTPD3 (Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3))

    Andere Bezeichnung

    CD39L3

    Gen-ID

    956

    UniProt

    O75355
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