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INA ELISA Kit
INA
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4881780
Produktdetails INA ELISA Kit
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Target
Alle INA ELISA Kits anzeigen
INA
(Internexin Neuronal Intermediate Filament Protein, alpha (INA))
Reaktivität
Alle Reaktivitäten für INA ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human Alpha-Internexin ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Human C3a.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu INA
(ausblenden)
Target
Alle INA ELISA Kits anzeigen
INA
(Internexin Neuronal Intermediate Filament Protein, alpha (INA))
Andere Bezeichnung
alpha-Internexin (INA Produkte )
Synonyme
NEF5 ELISA Kit, NF-66 ELISA Kit, TXBP-1 ELISA Kit, Inexa ELISA Kit, Intlaa ELISA Kit, Nf66 ELISA Kit, AV028420 ELISA Kit, NF66 ELISA Kit, ina-A ELISA Kit, xefiltin ELISA Kit, ina-a ELISA Kit, inexa ELISA Kit, MGC82496 ELISA Kit, INA ELISA Kit, si:ch211-67n3.6 ELISA Kit, nef5 ELISA Kit, xina ELISA Kit, nf-66 ELISA Kit, txbp-1 ELISA Kit, MGC69277 ELISA Kit, gefiltin ELISA Kit, zgc:110516 ELISA Kit, internexin neuronal intermediate filament protein alpha ELISA Kit, internexin neuronal intermediate filament protein, alpha ELISA Kit, internexin neuronal intermediate filament protein alpha S homeolog ELISA Kit, low molecular weight neuronal intermediate filament S homeolog ELISA Kit, internexin neuronal intermediate filament protein, alpha a ELISA Kit, internexin neuronal intermediate filament protein, alpha b ELISA Kit, INA ELISA Kit, Ina ELISA Kit, ina.S ELISA Kit, nif.S ELISA Kit, inaa ELISA Kit, ina ELISA Kit, inab ELISA Kit
Gen-ID
9118
UniProt
Q16352
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