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IGFBP2 ELISA Kit
IGFBP2
Reaktivität: Cat
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4881653
Produktdetails IGFBP2 ELISA Kit
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Target
Alle IGFBP2 ELISA Kits anzeigen
IGFBP2
(Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
Reaktivität
Alle Reaktivitäten für IGFBP2 ELISA Kits
Cat
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Feline (cat) Insulin-like Growth Factor Binding Protein 2 (IGFBP2) ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes Feline Insulin-like Growth Factor Binding Protein 2 (IGFBP2)
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu IGFBP2
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Target
Alle IGFBP2 ELISA Kits anzeigen
IGFBP2
(Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
Andere Bezeichnung
IGFBP-2 (IGFBP2 Produkte )
Synonyme
IBP2 ELISA Kit, IGF-BP53 ELISA Kit, AI255832 ELISA Kit, IBP-2 ELISA Kit, Igfbp-2 ELISA Kit, mIGFBP-2 ELISA Kit, IGFBP-2 ELISA Kit, ILGFBPA ELISA Kit, pIGFBP-2 ELISA Kit, igfbp2 ELISA Kit, MGC65741 ELISA Kit, MGC76823 ELISA Kit, MGC174445 ELISA Kit, IGFBP2 ELISA Kit, ibp2 ELISA Kit, igf-bp53 ELISA Kit, igfbp2a ELISA Kit, si:ch211-2k18.3 ELISA Kit, insulin like growth factor binding protein 2 ELISA Kit, insulin-like growth factor binding protein 2 ELISA Kit, insulin-like growth factor binding protein 2a ELISA Kit, insulin-like growth factor binding protein 2b ELISA Kit, IGFBP2 ELISA Kit, Igfbp2 ELISA Kit, igfbp2a ELISA Kit, igfbp2 ELISA Kit, igfbp2b ELISA Kit
UniProt
Q66WU6
Pathways
Myometrial Relaxation and Contraction , Growth Factor Binding , Activated T Cell Proliferation
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