Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

RAGE ELISA Kit

Hund RAGE ELISA-Kit Colorimetric-Assay zur Quantifizierung von Hund RAGE in Plasma, Cell Culture Supernatant und Serum
Produktnummer ABIN4881543

Kurzübersicht für RAGE ELISA Kit (ABIN4881543)

Target

Alle RAGE (AGER) ELISA Kits anzeigen
RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Reaktivität

  • 7
  • 7
  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Hund

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Applikation

ELISA

Proben

Plasma, Cell Culture Supernatant, Serum
  • Verwendungszweck

    Canine RAGE ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

    Analytische Methode

    Quantitative

    Spezifität

    This ELISA antibody pair recognizes Canine RAGE.

    Produktmerkmale

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Benötigtes Material

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    -20 °C

    Informationen zur Lagerung

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Haltbarkeit

    6 months
  • Target Alle RAGE (AGER) ELISA Kits anzeigen

    RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

    Andere Bezeichnung

    RAGE

    UniProt

    Q24LN3

    Pathways

    Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteine
Sie sind hier:
Chat with us!