FIL1d ELISA Kit
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- Target Alle FIL1d ELISA Kits anzeigen
- FIL1d (Interleukin 1 delta (FIL1d))
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Reaktivität
- Rind (Kuh)
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Custom Bovine IL-1 F5 (IL36RN/FIL1 delta) ELISA Kit.
- Proben
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytische Methode
- Quantitative
- Spezifität
- The antibody pair provided in this kit recognizes Bovine IL-1 F5 (IL36RN/FIL1 delta)
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle FIL1d ELISA Kits anzeigen
- FIL1d (Interleukin 1 delta (FIL1d))
- Andere Bezeichnung
- IL-36 Ra (FIL1d Produkte)
- Synonyme
- AI413231 ELISA Kit, Fil1delta ELISA Kit, IL36RN ELISA Kit, Il-1h3 ELISA Kit, Il1hy1 ELISA Kit, FIL1 ELISA Kit, FIL1(DELTA) ELISA Kit, FIL1D ELISA Kit, IL1F5 ELISA Kit, IL1HY1 ELISA Kit, IL1L1 ELISA Kit, IL1RP3 ELISA Kit, IL36RA ELISA Kit, PSORP ELISA Kit, Il1f5 ELISA Kit, interleukin 1 family, member 5 (delta) ELISA Kit, interleukin 36 receptor antagonist ELISA Kit, Il1f5 ELISA Kit, IL36RN ELISA Kit, Il36rn ELISA Kit
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