Substance P ELISA Kit

Produktnummer ABIN4881333

Maus, Human und Ratte Substance P ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus, Human und Ratte Substance P.

Kurzübersicht für Substance P ELISA Kit (ABIN4881333)

Target: Substance P

Reaktivität: Maus, Human, Ratte

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.1 ng/mL - 1000 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Substance P ELISA Kit

Untere Nachweisgrenze: 0.1 ng/mL

Verwendungszweck: Human/Mouse/Rat Substance P EIA Kit optimized for serum, plasma, and cell culture supernatants. Competition-based ELISA

Analytische Methode: Quantitative

Spezifität: This EIA kit is designed to detect human, mouse, and rat Substance P.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: Human: 2x / Mouse: 8x / Rat: 8X

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Haltbarkeit: 6 months

Details zu Substance P

Target: Substance P

Hintergrund:

Gene Symbols: TAC1, NKA, NKNA, TAC2

Background: Substance P (SP) is an 11 amino acid peptide produced from a precursor protein, Protachykinin-1. It is a neuropeptide, acting as a neurotransmitter and as a neuromodulator. It is found in the brain and spinal cord and is associated with inflammatory processes and pain. Its receptor, NK1R, is distributed over cytoplasmic and nuclear membranes of many cell types (neurons, glia, endothelia of capillaries and lymphatics, fibroblasts, stem cells, white blood cells) in many tissues and organs. Substance P has been found as a key first responder to most noxious/extreme stimuli (stressors). It has been reported that Substance P is involved in many biological events, such as vasodilation, inflammation, pain, mood, anxiety, Learning, vomiting, cell growth, proliferation, angiogenesis, and migration. Elevation of Substance P and/or its receptor (NK1R) in serum, plasma, or tissue has been associated with many diseases.

Protein Name (Synonyms): Protachykinin-1 (PPT) [Cleaved into: Substance P Neurokinin A (NKA) (Neuromedin L) (Substance K) Neuropeptide K (NPK) Neuropeptide gamma C-terminal-flanking peptide]

Gen-ID: 6863

UniProt: P20366