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NUCB2 ELISA Kit

NUCB2 Reaktivität: Ratte, Human, Maus Colorimetric Competition ELISA 0.1-1.000 ng/mL Cell Culture Supernatant, Serum
Produktnummer ABIN4881318
  • Target Alle NUCB2 ELISA Kits anzeigen
    NUCB2 (Nucleobindin 2 (NUCB2))
    Reaktivität
    Ratte, Human, Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Competition ELISA
    Detektionsbereich
    0.1-1.000 ng/mL
    Untere Nachweisgrenze
    0.1 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human/Mouse/Rat Nucleobindin-2 EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
    Proben
    Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This kit detects 420aa Nucleobindin-2 protein.

    Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Angiotensin II, NPY and APC.
    Sensitivität
    147 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samplesHuman: 4X / Mouse: 4X / Rat: 2X
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    Haltbarkeit
    6 months
  • Target Alle NUCB2 ELISA Kits anzeigen
    NUCB2 (Nucleobindin 2 (NUCB2))
    Andere Bezeichnung
    Nucleobindin-2 (NUCB2 Produkte)
    Synonyme
    nefa ELISA Kit, MGC97715 ELISA Kit, NUCB2 ELISA Kit, nucleobindin-2 ELISA Kit, NEFA ELISA Kit, AI607786 ELISA Kit, Calnuc ELISA Kit, Nefa ELISA Kit, Nesfatin-1 ELISA Kit, p54 ELISA Kit, nucleobindin 2 ELISA Kit, nucleobindin 2 L homeolog ELISA Kit, nucb2 ELISA Kit, NUCB2 ELISA Kit, Nucb2 ELISA Kit, nucb2.L ELISA Kit
    Gen-ID
    4925
    UniProt
    P80303
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