Chromogranin A ELISA Kit

Produktnummer ABIN4881303

Human, Maus und Ratte Chromogranin A ELISA Kit, Colorimetric Assay für die Quantifizierung von Human, Maus und Ratte Chromogranin A.

Kurzübersicht für Chromogranin A ELISA Kit (ABIN4881303)

Target: Chromogranin A (CHGA)

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.1 ng/mL - 1000 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Chromogranin A ELISA Kit

Untere Nachweisgrenze: 0.1 ng/mL

Verwendungszweck: Human/Mouse/Rat Catestatin EIA Kit optimized for serum, plasma and cell culture supernatants. Competition-based ELISA

Analytische Methode: Quantitative

Spezifität: This EIA kit is designed to detect human, mouse, and rat Catestatin.

Sensitivität: 0.5 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: Human: 12X / Mouse: 12X / Rat: 8X

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Standard, Biotinylated Catestatin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Haltbarkeit: 6 months

Details zu Chromogranin A

Target: Chromogranin A (CHGA)

Andere Bezeichnung: Catestatin

Hintergrund:

Gene Symbols: CHGA

Background: Catestatin is a 21-amino acid peptide that is cleaved from its precursor chromogranin A. As a potent nicotinic cholinergic antagonist, Catestatin inhibits catecholamine release from chromaffin cells and noradrenergic neurons. It also can induce mast cell migration, degranulation and stimulate histamine release. It has been suggested that early deficiency of Catestatin might play a pathogenic role in the subsequent development of the hypertension.

Catestatin also acts on innate immunity, and shows antibacterial activity against Gram-positive and Gram-negative bacteria.

Protein Name (Synonyms): Chromogranin-A (CgA) (Pituitary secretory protein I) (SP-I) [Cleaved into: Vasostatin-1 (Vasostatin I) Vasostatin-2 (Vasostatin II) EA-92 ES-43 Pancreastatin SS-18 WA-8 WE-14 LF-19 AL-11 GV-19 GR-44 ER-37]

Gen-ID: 1113

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling