Amylin/DAP ELISA Kit

Produktnummer ABIN4881297

Human, Maus und Ratte Amylin/DAP ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human, Maus und Ratte Amylin/DAP.

Kurzübersicht für Amylin/DAP ELISA Kit (ABIN4881297)

Target: Amylin/DAP (IAPP) (Islet Amyloid Polypeptide (IAPP))

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.1-1.000 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails Amylin/DAP ELISA Kit

Untere Nachweisgrenze: 0.1 ng/mL

Verwendungszweck: Human/Mouse/Rat Amylin (IAPP) EIA Kit optimized for serum and cell culture medium. Competition-based ELISA on a 96-well strip plate.

Analytische Methode: Quantitative

Spezifität: Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.

Sensitivität: 0.62 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Standard, Biotinylated Amylin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Haltbarkeit: 6 months

Details zu Amylin/DAP

Target: Amylin/DAP (IAPP) (Islet Amyloid Polypeptide (IAPP))

Andere Bezeichnung: Amylin

Gen-ID: 3375

UniProt: P10997

Pathways: Hormone Activity, Feeding Behaviour