EBV VCA IgM ELISA Kit

Produktnummer ABIN459379

Human EBV VCA IgM ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human EBV VCA IgM.

Kurzübersicht für EBV VCA IgM ELISA Kit (ABIN459379)

Target: EBV VCA IgM (Anti-Epstein-Barr Virus Viral Capsid Antigen IgM (EBV VCA IgM))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Serum

Produktdetails EBV VCA IgM ELISA Kit

Verwendungszweck: ELISA-VIDITEST anti-VCA EBV IgM assay is a solid-phase immunoanalytical test.

Marke: ELISA-VIDITEST

Analytische Methode: Semi-Quantitative

Bestandteile:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 2.0 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 13 mL Anti-human IgM animal antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ-V 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• 2 mL RF-sorbent (anti-human IgG) 25 x concentrated RF SORB 25x 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Benötigtes Material:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: anti-VCA EBV IgM assay is a solid-phase immunoanalytical test. The strips are coated with specific antigen that bears immunodominant epitopes of VCA complex. If relevant antibodies are present in the test samples, they bind to the immobilized antigen. The bound antibodies then in a next step react with horseradish peroxidase-labeled anti-human IgM antibodies. The amount of labeled antibodies bound is determined by the color enzymatic reaction. Negative samples do not react, a mild change in the colour of the wells is the reaction background.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu EBV VCA IgM

Target: EBV VCA IgM (Anti-Epstein-Barr Virus Viral Capsid Antigen IgM (EBV VCA IgM))

Substanzklasse: Antibody