EBV VCA IgA ELISA Kit

Produktnummer ABIN459374

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human EBV VCA IgA.

Kurzübersicht für EBV VCA IgA ELISA Kit (ABIN459374)

Target: EBV VCA IgA (Anti-Epstein-Barr Virus Viral Capsid Antigen IgA (EBV VCA IgA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Serum

Produktdetails EBV VCA IgA ELISA Kit

Verwendungszweck: anti-VCA EBV IgA is a solid-phase immunoanalytical test.

Marke: ELISA-VIDITEST

Analytische Methode: Semi-Quantitative

Bestandteile:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 2 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 13 mL Anti-human IgA animal antibodies labelled with horseradish peroxidase (anti-IgA Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H 2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Benötigtes Material:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: anti-VCA EBV IgA is a solid-phase immunoanalytical test. A specific antigen representing immunodominant epitopes of the VCA complex is bound to the surface of the wells. If antibodies are present in the test samples, they will bind to the immobilized proteins. The bound antibodies then react in the next step with horseradish peroxidase-labeled anti-human IgA antibodies. The amount of bound labeled antibodies is determined by a color enzymatic reaction. Negative samples do not react, a slight change in the color of the wells is the background of the reaction.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu EBV VCA IgA

Target: EBV VCA IgA (Anti-Epstein-Barr Virus Viral Capsid Antigen IgA (EBV VCA IgA))

Substanzklasse: Antibody