EBV NA IgM ELISA Kit

Produktnummer ABIN459362

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human EBV NA IgM.

Kurzübersicht für EBV NA IgM ELISA Kit (ABIN459362)

Target: EBV NA IgM (Anti-Epstein-Barr Virus Nuclear Antigen IgM (EBV NA IgM))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails EBV NA IgM ELISA Kit

Verwendungszweck: anti-EBNA-1 EBV IgM is an enzyme linked imunosorbent assay.

Marke: ELISA-VIDITEST

Analytische Methode: Semi-Quantitative

Bestandteile:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 1.3 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 13 mL Anti-human IgM animal antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H 2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Benötigtes Material:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: anti-EBNA-1 EBV IgM is an enzyme linked imunosorbent assay. Reaction wells are coated with the nuclear antigen containing immunodominant epitopes of EBNA-1. Its immunoreactivity is increased by conjugation to a protein carrier. Anti-EBNA-1 EBV antibodies from serum samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away. If antibodies are present in the test samples, they will bind to the immobilized peptide. The bound antibodies then react in the next step with horseradish peroxidase-labeled anti-human IgM antibodies. The amount of bound labeled antibodies is determined by a color enzymatic reaction. Negative samples do not react, a slight change in the color of the wells is the background of the reaction.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu EBV NA IgM

Target: EBV NA IgM (Anti-Epstein-Barr Virus Nuclear Antigen IgM (EBV NA IgM))

Substanzklasse: Antibody