EBV EA(D) IgG ELISA Kit

Produktnummer ABIN459354

Dieses Human EBV EA(D) IgG ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human EBV EA(D) IgG zu quantifizieren.

Kurzübersicht für EBV EA(D) IgG ELISA Kit (ABIN459354)

Target: EBV EA(D) IgG (Anti-Epstein-Barr Virus Early Antigen (D) IgG (EBV EA(D) IgG))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails EBV EA(D) IgG ELISA Kit

Verwendungszweck: anti-EA (D)EBV IgG assay is a solid-phase immunoanalytical test.

Marke: ELISA-VIDITEST

Analytische Methode: Semi-Quantitative

Bestandteile:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 1.3 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O 2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Benötigtes Material:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: anti-EA (D)EBV IgG assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a specific recombinant antigen containing immunodominant epitopes of EA antigen. Anti-EA (D) antibodies from samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away those that formed complexes with the antigens are later on recognized by animal anti-human IgG antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative samples do not react and the mild change in colour, if present, may be attributed to the reaction background.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu EBV EA(D) IgG

Target: EBV EA(D) IgG (Anti-Epstein-Barr Virus Early Antigen (D) IgG (EBV EA(D) IgG))

Andere Bezeichnung: Anti-EA (D) Epstein-Barr Virus (EBV) IgG Antibody

Substanzklasse: Antibody