IgG ELISA Kit

Produktnummer ABIN457295

Produktdetails IgG ELISA Kit

Target: IgG

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Untere Nachweisgrenze: 15.6 ng/mL

Applikation: ELISA

Verwendungszweck: The IgG(bovine)-ELISA is designed for the quantitative determination of bovine IgG in complex samples.

Analytische Methode: Quantitative

Spezifität: IgG (mouse) <0.1%

Produktmerkmale: The determination of bovine IgG is carried out as direct sandwich ELISA. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum has been used in the production process.

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: An antibody specific for bovine IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.

Testdurchführung:

Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm

Testpräzision: Intraassay precision (CV): (n=12) at 35 ng/ml: 2.9% at 112 ng/ml: 5.4%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IgG

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody