Anti-Alpha1 Adrenergic Receptor Antibodies (IgG) ELISA Kit

Produktnummer ABIN457293

Das Human Anti-Alpha1 Adrenergic Receptor Antibodies (IgG) ELISA Kit (ABIN457293) ist ein Colorimetric ELISA Kit zur Detektion von Human Anti-Alpha1 Adrenergic Receptor Antibodies (IgG).

Kurzübersicht für Anti-Alpha1 Adrenergic Receptor Antibodies (IgG) ELISA Kit (ABIN457293)

Target: Anti-Alpha1 Adrenergic Receptor Antibodies (IgG)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Produktdetails

Analytische Methode: Quantitative

Anwendungsinformationen

Plattentyp: Pre-coated

Testdurchführung:

Incubation of samples/ standards/ controls: 100 µL 120 min, 4°C
Wash Incubation of detection antibody: 100 µL 1 hr, room temperature
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add Stopp solution: 100 µL/well
Read at 450nm

Testpräzision: Intraassay-Precision: 8.77% Interassay-Precision: 11.83%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Antigendetails

Target: Anti-Alpha1 Adrenergic Receptor Antibodies (IgG)

Substanzklasse: Antibody

Hintergrund: The Alpha-1 adrenergic receptor is a G protein-coupled receptor. It consists of three highly homologous subtypes, including a1A-, a1B-, and a1D-adrenergic. Catecholamines like noradrenaline and adrenaline signal through the a1-adrenergic receptor in the central and peripheral nervous systems. The occurrence of auto- antibodies against (1-adrenergic-receptor is associated with the existence of malignant hypertension. The anti-(1-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the (1-adrenergic-receptor in serum and plasma. _x000E_ Principle of the test The anti-(1-adrenergic-receptor-antibody-EIA is an antibody screening test. The (1-adrenergic-receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-(1-adrenergic-receptor-antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-(1-adrenergic-receptor-antibodies.