CRABP2 ELISA Kit

Produktnummer ABIN455486

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human CRABP2.

Kurzübersicht für CRABP2 ELISA Kit (ABIN455486)

Target: CRABP2 (Cellular Retinoic Acid Binding Protein 2 (CRABP2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312-20 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails CRABP2 ELISA Kit

Untere Nachweisgrenze: 0.312 ng/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of human IFN?-1b concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Spezifität: This assay recognizes recombinant and natural human IFN?-1b.

Kreuzreaktivität (Details): No significant cross-reactivity or interference was observed.

Sensitivität: < 3.9 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Produktmerkmale: Homo sapiens,Human,Cellular retinoic acid-binding protein 2,Cellular retinoic acid-binding protein II,CRABP-II,CRABP2

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFNα-1b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IFNα-1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IFNα-1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IFNα-1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu CRABP2

Target: CRABP2 (Cellular Retinoic Acid Binding Protein 2 (CRABP2))

Andere Bezeichnung: CRABP2