Haptoglobin ELISA Kit

Produktnummer ABIN455286

Das Rind (Kuh) Haptoglobin ELISA Kit (ABIN455286) ist ein Colorimetric ELISA Kit zur Detektion von Rind (Kuh) Haptoglobin.

Kurzübersicht für Haptoglobin ELISA Kit (ABIN455286)

Target: Haptoglobin (HP)

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 μg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Haptoglobin ELISA Kit

Untere Nachweisgrenze: 0.78 μg/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of bovine Hpt concentrations in serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Spezifität: This assay recognizes recombinant and natural bovine Hpt.

Kreuzreaktivität (Details): No significant cross-reactivity or interference was observed.

Sensitivität: < 0.195 μg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Produktmerkmale: Bos taurus,Bovine,Haptoglobin,HP,Zonulin

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hpt. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hpt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hpt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Hpt in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu Haptoglobin

Target: Haptoglobin (HP)

Andere Bezeichnung: HP

Hintergrund: Haptoglobin (Hpt) is a plasma protein with hemoglobin-binding capacity, and a plasma glycoproteins that form a stable complex with hemoglobin to aid the recycling of heme iron. It is a well-known marker of hemolysis. High haptoglobin level in plasma was associated with an increased cardiovascular risk in obese men, inflammation, atherosclerosis, and systemic sclerosis.

Pathways: Transition Metal Ion Homeostasis