CCL24 ELISA Kit

Produktnummer ABIN455041

Maus CCL24 ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus CCL24.

Kurzübersicht für CCL24 ELISA Kit (ABIN455041)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Produktdetails CCL24 ELISA Kit

Verwendungszweck: This immunoassay kit allows for the specific measurement of mouse Eotaxin-2 (CCL24) concentrations in cell culture supernates, serum, plasma , BALF and tissue homogenates.

Analytische Methode: Quantitative

Spezifität: This assay recognizes recombinant and natural mouse CCL24. The factors listed below were assayed for cross-reactivity.

Kreuzreaktivität (Details): No significant cross-reactivity or interference was observed.

Produktmerkmale: Mus musculus,Mouse,C-C motif chemokine 24,Eosinophil chemotactic protein 2,Eotaxin-2,Small-inducible cytokine A24,Ccl24,Scya24

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CCL24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL24 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for CCL24 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL24 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu CCL24

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Andere Bezeichnung: Ccl24

Hintergrund: Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and Il-4. LPS and IL-4 also differentially regulate the expression of eotaxin-2 in monocytes and macrophages. Mouse eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human eotaxin-2. Functionally, eotaxin-2 is most closely related to eotaxin/CCL11 and eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin.

Pathways: Regulation of Actin Filament Polymerization