LDHD ELISA Kit

Produktnummer ABIN454979

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus LDHD.

Kurzübersicht für LDHD ELISA Kit (ABIN454979)

Target: LDHD (Lactate Dehydrogenase D (LDHD))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Serum, Tissue Homogenate

Produktdetails LDHD ELISA Kit

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Mouse D-LDH, concentrations in serum, tissue homogenates and other biological fluids.

Analytische Methode: Quantitative

Spezifität: This assay recognizes recombinant and natural Mouse D-LDH.

Kreuzreaktivität (Details): No significant cross-reactivity or interference was observed.

Sensitivität: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Produktmerkmale: Mus musculus,Mouse,Probable D-lactate dehydrogenase, mitochondrial,DLD,Lactate dehydrogenase D,Ldhd,1.1.2.4

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-LDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for D-LDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of D-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu LDHD

Target: LDHD (Lactate Dehydrogenase D (LDHD))

Andere Bezeichnung: Ldhd

Pathways: Ribonucleoside Biosynthetic Process