HMGB4 ELISA Kit

Produktnummer ABIN454716

Dieses Human HMGB4 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human HMGB4 zu quantifizieren.

Kurzübersicht für HMGB4 ELISA Kit (ABIN454716)

Target: HMGB4 (High Mobility Group Box 4 (HMGB4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156-10 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails HMGB4 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of human chondroitin sulfatase,CS concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Spezifität: This assay recognizes recombinant and natural human CS.

Kreuzreaktivität (Details): No significant cross-reactivity or interference was observed.

Sensitivität: < 0.312 U/L
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Produktmerkmale: Homo sapiens,Human,High mobility group protein B4,HMGB4

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu HMGB4

Target: HMGB4 (High Mobility Group Box 4 (HMGB4))

Andere Bezeichnung: HMGB4

Hintergrund: In enzymology, a chondroitin sulfatase is an enzyme that catalyzes the chemical reaction of cleaving off the 6-sulfate groups of the N-acetyl-D-galactosamine 6-sulfate units of the macromolecule chondroitin sulfate and, similarly, of the D-galactose 6-sulfate units of the macromolecule keratan sulfate.This enzyme belongs to the family of hydrolases, specifically those acting on sulfuric ester bonds.