Anti-PAR1 Antibody (IgG) ELISA Kit

Produktnummer ABIN454033

Produktdetails

Target: Anti-PAR1 Antibody (IgG)

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Verwendungszweck: The anti-alpha2-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the Alpha2-adrenergic-receptor in serum and plasma.

Analytische Methode: Quantitative

Produktmerkmale: The anti-Thrombin-Receptor-Antibody-EIA is an antibody screening test.

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: The Thrombin-Receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-Thrombin-Receptor-Antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-Thrombin-Receptor-Antibody.

Testdurchführung:

Incubation of samples/ standards/ controls: 100 µL 120 min, 4°C
Wash Incubation of detection antibody: 100 µL 1 hr, room temperature
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add Stopp solution: 100 µL/well
Read at 450nm

Testpräzision: Intraassay-Precision:7,83% Interassay-Precision:9,12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Antigendetails

Target: Anti-PAR1 Antibody (IgG)

Substanzklasse: Antibody

Hintergrund: The Alpha-2 adrenergic receptor is a G protein-coupled receptor. ß receptors have the subtypes ß1, ß2 and ß3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP.