IgG ELISA Kit
Kurzübersicht für IgG ELISA Kit (ABIN454029)
Target
Alle IgG ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
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Untere Nachweisgrenze
- 156 ng/mL
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Verwendungszweck
- The IgG(rat)-ELISA is designed for the quantitative determination of rat IgG in complex samples (serum or other biological samples).
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Analytische Methode
- Quantitative
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Produktmerkmale
- The determination of rat IgG is carried out as direct sandwich ELISA.
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IgG ELISA Kit
Reaktivität: Kaninchen Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP Reaktivität: Kaninchen
Mouse TrueBlot® Set (with IP beads)WB, IP Reaktivität: Maus
IgG ELISA KitReaktivität: Maus Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReaktivität: Schwein Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReaktivität: Maus Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReaktivität: Human Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReaktivität: Huhn Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReaktivität: Rind (Kuh) Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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Plattentyp
- Pre-coated
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Protokoll
- An antibody specific for rat IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
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Testdurchführung
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm -
Testpräzision
- Intraassay precision (CV): (n=10) at 73 ng/ml: 5.8% at 274 ng/ml: 8.9%
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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- IgG
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Substanzklasse
- Antibody
Target Alle IgG ELISA Kits anzeigen
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