IgG ELISA Kit

Produktnummer ABIN454028

Produktdetails IgG ELISA Kit

Target: IgG

Reaktivität: Kaninchen

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Untere Nachweisgrenze: 156 ng/mL

Applikation: ELISA

Verwendungszweck: The IgG(rabbit)-ELISA is designed for the quantitative determination of rabbit IgG in complex samples (serum or other biological samples).

Proben: Serum

Analytische Methode: Quantitative

Produktmerkmale: The determination of rabbit IgG is carried out as direct sandwich ELISA.

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: An antibody specific for rabbit IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.

Testdurchführung:

Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm

Testpräzision: Intraassay precision (CV):(n=10)at 4.1 ng/ml: 2.9%at 17.3 ng/ml: 2.5%at 34.7 ng/ml: 2.6%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IgG

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody