IgG ELISA Kit

Produktnummer ABIN454027

Schwein IgG ELISA Kit, Colorimetric Assay für die Quantifizierung von Schwein IgG.

Kurzübersicht für IgG ELISA Kit (ABIN454027)

Target: IgG

Reaktivität: Schwein

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Serum

Produktdetails IgG ELISA Kit

Untere Nachweisgrenze: 156 ng/mL

Verwendungszweck: The IgG(pig)-ELISA is designed for the quantitative determination of pig IgG in complex samples (serum or other biological samples).

Analytische Methode: Quantitative

Produktmerkmale: The determination of pig IgG is carried out as direct sandwich ELISA.

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: An antibody specific for pig IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.

Testdurchführung:

Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm

Testpräzision: Intraassay precision (CV): (n=10) at 57 ng/ml: 5.3% at 218 ng/ml: 2.7% at 580 ng/ml: 4.7%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IgG

Target: IgG

Substanzklasse: Antibody